Isolation of Magnetotatic Bacteria from a Graphite Mine in Upstate New York

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Issue Date
2015-04-10
Authors
Monroe, Jeremy G.
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Keywords
Magnetotactic Bacteria , Isolated , New Habitat
Abstract
Magnetotactic bacteria have the ability to orient and migrate along geomagnetic field lines. This unique ability is due to specific intracellular organelles called magnetosomes, which are membrane bound crystals of iron oxides called magnetite oriented linearly inside the bacterial cells. The properties of magnetotactic bacteria are of increasing interest in many fields, including environmental science and biogeology, and bacterial magnetites have proven useful as tools in both biological research and medicine. A sample of water from a graphite mine in Hague, New York was analyzed for the presence of magnetotatic bacteria. Potential magnetotatic bacteria were concentrated using a neodymium magnet attached to the sample. The concentrated sample was then isolated using the streak plate method on a ferric quinate agar medium incubated at 25°C. The isolated colonies were then tested for magnetotaxis by observing the movement of bacteria in the presence of a magnetic field. A magnetotatic bacterium was isolated using this method and presumptively identified as Aquaspirillum magnetotacticum. The presence of magnetotatic bacteria in an area of high nonmagnetic material shows the wide environmental range of these bacteria. Previously magnetotatic bacteria had only been isolated from areas containing high amounts of magnetic materials such as pond or sea water. This research expands the range of possible habitats for Aquaspirillum magnetotacticum, and demonstrates the ability of this bacterium to adapt to environments that do not provide large concentrations of the materials needed to maintain magnetotaxis.
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