Inducing RNAi by feeding
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Authors
Guan, Jinming
Jiang, Yunru
Yang, Siyuan
Issue Date
2015-04-10
Type
poster_presentation
Language
en_US
Keywords
RNAi , C. Elegans , Gene Silence
Alternative Title
Abstract
RNA interference (RNAi) is a technique that deliberately introducing dsRNA sequences identical to the sequence of a gene, then the result of the silence of the gene can be observed. RNAi can be activated in C. elegans, a roundworm commonly studied by researchers, by feeding worms bacteria expressing dsRNA. In this project, two RNAi strain of E. coli, dqy-11 and bli-1, will be introduced to C. elegans. Each RNAi “feeding strain” of bacteria contains a plasmid with a piece of DNA containing sequence from the gene it is designed to silence. The dsRNA eaten by the worms will turn down the gene and the results can be examined by any changes of the phenotypes.