Development of high yield Switchgrass Cultivar by Agrobacterium-Mediated transformation with InsP-5-Ptase Gene
McMaster-Schuyler, Lynda, Dr.
Zeng, Peiyu, Dr.
InsP-5-Ptase gene is involved in the phosphoinostide pathway which functions as a mechanism of drought response. The protein products of this gene hydrolyze soluble inositol phosphates terminating the signal of InsP3 triggered by abscisic acid, a chemical produced in large quantities during drought. Switchgrass is an important warm season grass in Northern America, often used in the production of biofuels. By manipulating the switchgrass genome, we can increase the yield of desired products, including ethanol. Introducing the InsP-5-Ptase gene into the callus will give the plants an ability to grow in arid soil, making it a very valuable biofuel crop. In this research, we are overexpressing drought tolerance gene coding InsP-5-Ptase in switchgrass plants by agrobacterium-mediated transformation method. The binary vector pHL083 containing gene expression cassettes of InsP-5-Ptase and herbicide-resistant Bar gene. Transgenic herbicide resistant switchgrass plants have been obtained. Further molecular, physiological and progeny analysis will be conducted.