Using Bioinformatics Tools to Study the Hypothetical Protein Rho Guanine Nucleotide Exchange Factor 37
Journal Title
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Issue Date
2022
Authors
Czajkowski, Hunter
Publisher
SUNY Brockport
Keywords
Abstract
In this study the hypothetical protein Rho guanine nucleotide exchange factor 37, was analyzed
through the use of four bioinformatics tools: SMART, GlobPlot, Phyre2, and BLAST. The
universal protein data bank, Uniprot, was used to select the hypothetical protein, while the
bioinformatics tools were used to investigate the hypothetical protein. SMART was used in the
identification and analysis of the protein domains within the Rho guanine nucleotide exchange
factor 37 protein sequence. GlobPlot was used to predict disorder and globular sequences of the
hypothetical protein. Phyre2 was used to build a three dimensional model of Rho guanine
nucleotide exchange factor 37, as well as determine the best matching known protein. Phyre
concluded that Rho guanine nucleotide exchange factor 16 was the closest match, and used this
data to produce a model with 100% confidence and 54% coverage for rho guanine nucleotide
exchange factor 37. BLAST was also used to find the most similar match to Rho guanine
nucleotide exchange factor 37, which produced a 98.8% to Rho guanine nucleotide factor 37
(Pan troglodytes). Using the bioinformatics tools, predictions regarding protein location and
function were concluded. The predicted location of Rho guanine nucleotide exchange factor 37 is
in the intracellular-cytosol, while it is predicted that Rho guanine nucleotide exchange factor 37
may act as a guanine nucleotide exchange factor which facilitates the release of guanosine
diphosphate (GDP) to allow binding of guanosine triphosphate. This study will overall
investigate the hypothetical protein Rho guanine nucleotide exchange factor 37 through the use
of the bioinformatics tools in order to best understand the hypothetical protein's function.
Description
FODL Library Research Awards
One of two 3rd-place winners for this year.
DOI
Content Designation
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