Soybean Transformation with Cysteine Protease Inhibitor (CPI1) Gene for Resistance and Tolerance

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Snare, Melissa
Cross, Janel
Zhou, Yeying
McMaster-Schuyler, Lynda, Dr.
Zeng, Peiyu, Dr.
Soybeans (Glycine max) are one of the major agricultural important crops in the United States. These legumes have a variety of uses, including food and industrial applications. Soybean growth is susceptible to drought, pest infection, and bacterial disease. These conditions strain soybean growth worldwide, dampening the effect that soybeans have on the economic network. It has been reported that the introduction of an active Cysteine Protease Inhibitor gene (CPI1) originally identified in Arabidopsis thaliana, will enhance soybean disease resistance and drought tolerance. The CPI1 gene helps the soybean combat programed cell death, which occurs when soybeans are exposed to unfavorable conditions and stress. Protease enzymes, released during cell death, break down the proteins and peptides necessary for the cell to live. Therefore, with the introduction of the CPI1 gene into soybean DNA protease levels can be controlled to help overcome programmed cell death. This will produce a heartier soybean that will more readily survive in a variety of adverse environmental conditions. The transgenic soybeans overexpressing CPI1 genes will grow transgenic soybeans that will promote soybean growth and its role in the economy. [2]