Inducing RNAi of dpy11 and bli-1 genes in C. elegans
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Authors
Jiang, Yunru.
Yang, Siyuan.
Guan, Jinming.
McMaster-Schuyler, Lynda, Dr.
Issue Date
2015-04
Type
Presentation
Language
en_US
Keywords
Alternative Title
Abstract
RNA interference (RNAi) is a biological process in which RNA molecules block gene expression, typically by causing the destruction of specific mRNA molecules. C. elegans is an excellent model system to study because many single gene mutations have been identified and the worms' transparency assist morphological observations. We are studying RNAi techniques by feeding E. coli to interrupt the dpy-11 and bli-1 genes in C. elegans, resulting in phenotype changes.
Description
In this research, RNAi constructs were first transformed in E. coli and C. elegans are fed E. coli bacteria containing relevant dsRNA for genes to be silenced. Phenotypic changes in offspring will be observed and documented to verify RNAi had been achieved.