• Login
    View Item 
    •   DSpace Home
    • SUNY Brockport
    • Events/Conferences
    • 2015 SUNY Undergraduate Research Conference
    • View Item
    •   DSpace Home
    • SUNY Brockport
    • Events/Conferences
    • 2015 SUNY Undergraduate Research Conference
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Validation of splice altering morpholino knockdown of anoctamin 1

    Thumbnail
    View/Open
    surc/2015/schedule/44/fulltext (1).pdf (17.36Kb)
    Date
    2015-04-10
    Author
    Denora, Maxwell
    Metadata
    Show full item record
    Subject
    Zebrafish
    Morpholino
    q-PCR
    Quantitative PCR
    Abstract
    Morpholino oligonucleotides (MO) are commonly used to reduce gene expression. MO selectivity is determined by selection of a unique oligonucleotide sequence in the target of interest Suitable targets for splice altering MO are at an intron-exon junction in pre messenger RNA. The MO is predicted to anneal to the complimentary sequence and to inhibit intron-exon splicing. However, it is possible that an MO may anneal to an un-identified target(s) resulting in a phenotype that is unrelated to the gene of interest. Therefore it is necessary to validate MO efficacy on the targeted gene. This objective for this project is to validate anoctamin 1 splice-altering MO efficacy in zebrafish. Quantitative polymerase chain reaction (Q-PCR) will be used to compare anoctamin 1 expression levels in non-injected control embryos and in MO-injected embryos at 2 and 5 days post fertilization (dpf). Anoctamin 1 splice altering MO was designed to excise exon 4. We predict that MO knockdown will be greater at 2 dpf compared to 5 dpf. Data will be presented validating primer design and efficacy. This work will contribute to a better understanding of the general MO mechanism of action and will also validate an important technique to study anoctamin 1 function in zebrafish.
    URI
    http://hdl.handle.net/1951/72877
    Collections
    • 2015 SUNY Undergraduate Research Conference [409]

    SUNY Digital Repository Support
    DSpace software copyright © 2002-2022  DuraSpace
    Contact Us | Send Feedback
    DSpace Express is a service operated by 
    Atmire NV
     

     


    SUNY Digital Repository Support
    DSpace software copyright © 2002-2022  DuraSpace
    Contact Us | Send Feedback
    DSpace Express is a service operated by 
    Atmire NV