Show simple item record

dc.contributor.authorBola, Ronald Aaron S.
dc.contributor.authorKrzyzanowski, Michelle C.
dc.contributor.authorWood, Jordan F.
dc.contributor.authorPortman, Douglas S.
dc.contributor.authorFerkey, Denise M.
dc.date.accessioned2021-09-07T19:23:50Z
dc.date.available2021-09-07T19:23:50Z
dc.date.issued2015-04-10
dc.identifier.urihttp://hdl.handle.net/1951/72680
dc.description.abstractBehavioral differences between sexes are evident across many species. The underlying mechanisms surrounding such differences are not fully elucidated, however, due to the complexities of animal behavior. The nematode Caenorhabditis elegans (C. elegans) is a well-characterized, genetically amenable species with two sexes, hermaphrodites (XX) and males (XO). This makes it an appropriate model system for investigating sex-based behavioral differences. Chemosensation in C. elegans is mediated by exposed ciliated sensory neurons, one of which is ASH. ASH is a polymodal nociceptor that elicits reversal when an animal encounters aversive stimuli. We hypothesized that hermaphrodite and male C. elegans worms respond differently to stimuli detected by ASH such as the bitter tastant quinine, the detergent sodium dodecyl sulfate (SDS), and the heavy metal copper (CuCl_2). Wild-type assay-age hermaphrodites and males were picked from a nematode growth media (NGM) plate with E. coli OP50 and kept on an NGM plate without food for 10 minutes prior to assaying. A drop of aversive stimulus was placed in front of a forward-moving animal, and the animal’s response was recorded. A positive response is backwards movement within 4 seconds after contact with the stimulus. Our results reveal a quantifiable difference in how wild-type hermaphrodite and male C. elegans respond to aversive stimuli. Specifically, wild-type males are less responsive than hermaphrodites to quinine, SDS, and CuCl_2. Further investigations will be conducted through experiments with C. elegans strains in which hermaphrodites have masculinized, and males have feminized nervous systems or subsets of neurons. Through these experiments, we aim to explore potential sites of difference that lead to these observable differences in responsiveness to aversive stimuli.
dc.language.isoen_US
dc.subjectC. Elegans
dc.subjectAnimal Behavior
dc.subjectBehavioral Differences
dc.subjectSensory Neurons
dc.subjectChemosensation
dc.subjectNociception
dc.titleSex-based Differences in C. elegans Responsiveness to Aversive Stimuli
dc.typeposter_presentation
dc.contributor.organizationUniversity at Buffalo, State University of New York
dc.contributor.organizationUniversity of Rochester
dc.description.institutionSUNY Brockport
dc.description.publicationtitleSUNY Undergraduate Research Conference
dc.source.statuspublished


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record