Coenzyme A plays a central role in metabolism as an important part of the TCA cycle and in the synthesis and oxidation of fatty acids. The biosynthesis of CoA is a highly conserved process in a broad range of organisms such as Kytococcus species. Kytococcus sedentarius is a gram positive, coccoid bacterium known to cause pitted keratolysis. The purpose of this study was to manually annotate computer called genes encoding proteins involved in the biosynthesis of coenzyme A from panthothenate in Kytococcus sedentarius. The annotation of each component of this biosynthetic pathway was done using the online Integrated Microbial Genome Annotation Toolkit (IMG-ACT). There were 4 computer called genes involved in the pathway. BLAST, multiple sequence alignment analysis and WebLog were used to verify homology with highly curated genes encoding proteins of the same function in other organisms. TMHMM and SignalP were used to predict the location of each protein in the cell. The reading frame predicted for each gene was verified and potential function of each protein was predicted using TIGRFAM and PFAM. Phylogenetic trees were constructed using the BLAST-based tool in the phylogeny.fr website and the evolutionary relationship of Kytococcus sedentarius with other organisms based on these gene sequences was analyzed. Our findings showed that all gene sequences involved in Coenzyme A synthesis in Kytococcus sendentarius were highly conserved. These analyses contribute to the consolidated curation of the Kytococcus sedentarius genome and to a better understanding of the evolutionary development and genetic composition of Kytococcus sedentarius.