Kytococcus sedentarius is an aerobic, gram positive, coccoid, non-endospore forming bacterium isolated from a marine environment. The genome of Kytococcus sedentarius is of great interest to annotate because of its biotechnological potential as a source of oligoketide antibiotics (monensin A and B), for its role in causing pitted keratolysis of the foot and in causing opportunistic infections. The genome has been sequenced and automated computer annotation of the genome has been performed. Manual annotation, however, holds great value because of documented inaccuracies involved in the computer annotation of the genomic database of the, as yet, lesser studied genome of Kytococcus sedentarius. The purpose of this study was to define and annotate the genes involved in the biosynthesis of chorismate in this organism. Chorismate is the direct precursor of many aromatic compounds and its biosynthesis occurs via the shikimate pathway only in bacteria, fungi, yeasts, algae, plants and certain parasites. Tools like BLAST, TMHMM, Phylogeny were used to annotate the genes with respect to the various characteristic features of the gene like amino acid sequence of the gene product, localization of the protein in the cell, structure, function, phylogenetic origin and pathways in which the gene product is involved. The annotation results suggested that gene for 3-dehydroquinate synthase was involved in 2 steps in the pathway. The gene encoding the enzyme 3-dehydroquinate dehydratase was proposed to have undergone a horizontal gene transfer from organisms like Clostridium based on results from the phylogenetic tree and gene neighborhood.