Characterization of Aminopeptidase Activities in Arabidopsis Thaliana Seedlings
PublisherMonroe Community College
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AbstractAminopeptidases are exopeptidases that are responsible for the removal of an amino acid residue from the N-terminus of peptides and protein substrates. The presence of a leucine aminopeptidase, an alanine aminopeptidase, and a proline iminopeptidase within plants had been hypothesized, and shown by previous proteonomic analysis. In order to test the hypothesis, the aim of the study was to determine whether aminopeptidase activity could be detected at the appropriate bands that had enzyme proteins. Extracts of 1-week and 3-week Arabidopsis Thaliana seedlings were examined. The activities found were characterized with respect to pH and substrate specificity using aminoacyl β-naphthylamine of proline, alanine, valine, and leucine.
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