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dc.contributor.authorTokarz, Rafal
dc.contributor.authorMishra, Nischay
dc.contributor.authorTagliafierro, Teresa
dc.contributor.authorSameroff, Stephen
dc.contributor.authorCaciula, Adrian
dc.contributor.authorChauhan, Lokendrasingh
dc.contributor.authorPatel, Jigar
dc.contributor.authorSullivan, Eric
dc.contributor.authorGucwa, Azad
dc.contributor.authorFallon, Brian
dc.contributor.authorGolightly, Marc
dc.contributor.authorGolightly, Marc
dc.contributor.authorMolins, Claudia
dc.contributor.authorSchriefer, Martin
dc.contributor.authorMarques, Adriana
dc.contributor.authorBriese, Thomas
dc.contributor.authorLipkin, W. Ian
dc.date.accessioned2018-10-03T18:26:09Z
dc.date.available2018-10-03T18:26:09Z
dc.date.issued2018-02-16
dc.identifier.issn2045-2322
dc.identifier.otherhttps://doi.org/10.1038/s41598-018-21349-2
dc.identifier.urihttp://hdl.handle.net/1951/70470
dc.descriptionThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en_US
dc.description.abstractTick-borne diseases are the most common vector-borne diseases in the United States, with serology being the primary method of diagnosis. We developed the first multiplex, array-based assay for serodiagnosis of tick-borne diseases called the TBD-Serochip. The TBD-Serochip was designed to discriminate antibody responses to 8 major tick-borne pathogens present in the United States, including Anaplasma phagocytophilum, Babesia microti, Borrelia burgdorferi, Borrelia miyamotoi, Ehrlichia chaffeensis, Rickettsia rickettsii, Heartland virus and Powassan virus. Each assay contains approximately 170,000 12-mer linear peptides that tile along the protein sequence of the major antigens from each agent with 11 amino acid overlap. This permits accurate identification of a wide range of specific immunodominant IgG and IgM epitopes that can then be used to enhance diagnostic accuracy and integrate differential diagnosis into a single assay. To test the performance of the TBD-Serochip, we examined sera from patients with confirmed Lyme disease, babesiosis, anaplasmosis, and Powassan virus disease. We identified a wide range of specific discriminatory epitopes that facilitated accurate diagnosis of each disease. We also identified previously undiagnosed infections. Our results indicate that the TBD-Serochip is a promising tool for a differential diagnosis not available with currently employed serologic assays for TBDs.en_US
dc.description.sponsorshipThis study was funded through a grants from the Steven and Alexandra Cohen Foundation, NIH/NIAID award U19 AI109761 - Lipkin, and the Calderone Junior Faculty Award – Tokarz.en_US
dc.language.isoen_USen_US
dc.publisherScientific Reportsen_US
dc.titleA multiplex serologic platform for diagnosis of tick-borne diseasesen_US
dc.typeArticleen_US


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