Hop (Humulus lupulus) is widely used in the brewing industry because the female flower improves the aroma and flavor of beer. It is challenging to cultivate hops in New York state since the hop is very sensitive to fungi and bacteria. Agene which could confer fungus resistance to hops would be useful. The goal of our project is to develop a tissue culture protocol which can be used to grow single cell hops capable of Agrobacterium-mediated transformation. We have established a tissue culture system for regeneration by using MS-B5 medium supplemented with 1.43µM IAA and 9.08µM TDZ, resulting in successful shoot induction. Small (0.5cm) shoots and green calli induced from shoot internodes in vitro have resulted in transplantable Hop.