Analysis of Transformation Effectiveness in Brassica rapa
Zeng, Peiyu, Dr.
McMaster-Schuyler, Lynda, Dr.
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Brassica rapa is an organism favored for its use as a model in plant biotechnology. Researchers at the University of Wisconsin-Madison selectively bred a cultivar of B. rapa that specifically serves this purpose, known as FPsc. Variations of this cultivar containing specific mutations served as the basis of this experiment. The efficiency of Agrobacterium mediated transformation via leaf disc infection was tested in B. rapa samples containing mutations of dwarfism and albinism as well as wild type and a strain of B. rapa known as R500 or yellow sarson. The goal of this experiment was not only to test the efficiency of Agrobacterium mediated transformation in these plants, but also how each mutation could possibly effect the outcome.