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dc.contributor.authorForaida, Zahraa I.
dc.contributor.authorSharikova, Anna
dc.contributor.authorPeerzada, Lubna N.
dc.contributor.authorKhmaladze, Alexander
dc.contributor.authorLarsen, Melinda
dc.contributor.authorCastracane, James
dc.creator
dc.date.accessioned2017-12-15T18:35:57Z
dc.date.available2017-12-15T18:35:57Z
dc.date.issued2017-08-29
dc.identifier.citationForaida, Z. I., Sharikova, A., Peerzada, L. N., Khmaladze, A., Larsen, M., & Castracane, J. (2017, August 29). Double emulsion electrospun nanofibers as a growth factor delivery vehicle for salivary gland regeneration. Proc. SPIE 10352, Biosensing and Nanomedicine X, 103520E. doi:10.1117/12.2275489en_US
dc.identifier.issn0277-786X
dc.identifier.urihttp://hdl.handle.net/1951/69523
dc.description.abstractSustained delivery of growth factors, proteins, drugs and other biologically active molecules is necessary for tissue engineering applications. Electrospun fibers are attractive tissue engineering scaffolds as they partially mimic the topography of the extracellular matrix (ECM). However, they do not provide continuous nourishment to the tissue. In search of a biomimetic scaffold for salivary gland tissue regeneration, we previously developed a blend nanofiber scaffold composed of the protein elastin and the synthetic polymer polylactic-co-glycolic acid (PLGA). The nanofiber scaffold promoted in vivo-like salivary epithelial cell tissue organization and apicobasal polarization. However, in order to enhance the salivary cell proliferation and biomimetic character of the scaffold, sustained growth factor delivery is needed. The composite nanofiber scaffold was optimized to act as a growth factor delivery system using epidermal growth factor (EGF) as a model protein. The nanofiber/EGF hybrid nanofibers were synthesized by double emulsion electrospinning where EGF is emulsified within a water/oil/water (w/o/w) double emulsion system. Successful incorporation of EGF was confirmed using Raman spectroscopy. EGF release profile was characterized using enzymelinked immunosorbent assay (ELIZA) of the EGF content. Double emulsion electrospinning resulted in slower release of EGF. We demonstrated the potential of the proposed double emulsion electrospun nanofiber scaffold for the delivery of growth factors and/or drugs for tissue engineering and pharmaceutical applications.en_US
dc.description.sponsorshipColleges of Nanoscale Science and Engineering, SUNY Polytechnic Institute Department of Physics, SUNY Albany Department of Biological Sciences, SUNY Albany National Institutes of Health, National Institute of Dental and Craniofacial Research R01DE022467 to M.L and J.C. National Institutes of Health C06 RR015464 to the University at Albany, SUNY National Science Foundation MRI award DBI0922830 to CNSE, SUNY Polytechnic Instituteen_US
dc.publisherSPIE Nanoscience + Engineeringen_US
dc.subjecttissue regenerationen_US
dc.subjectnanofibersen_US
dc.subjectepidermal growth factoren_US
dc.subjectgrowth factor deliveryen_US
dc.subjectRaman spectroscopyen_US
dc.titleDouble emulsion electrospun nanofibers as a growth factor delivery vehicle for salivary gland regenerationen_US
dc.title.alternativeProceedings of SPIE 10352, Biosensing and Nanomedicine Xen_US
dc.typePresentationen_US


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