• Login
    View Item 
    •   DSpace Home
    • SUNY Polytechnic Institute
    • Doctoral Dissertations
    • Colleges of Nanoscale Science and Engineering Doctoral Dissertations
    • View Item
    •   DSpace Home
    • SUNY Polytechnic Institute
    • Doctoral Dissertations
    • Colleges of Nanoscale Science and Engineering Doctoral Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Development of Novel Technologies for Direct Cellular Patterning for the Establishment of Well Controlled Microenvironments to Facilitate Studies on Cellular Signaling, Sensing, and Other Diffusion-Based Phenomena

    Thumbnail
    View/Open
    William Hynes Dissertation (2.266Mb)
    Hynes License (57.57Kb)
    Date
    2016-05-07
    Author
    Hynes, William
    Metadata
    Show full item record
    Subject
    bioprinting
    cellular signaling
    syntrophic relationships
    COMETS
    Abstract
    This work focuses on the utilization of novel bioprinting technologies for the investigation of cellular signaling, sensing, and other diffusion-based phenomena with spatiotemporal dependencies. Two different printing techniques were developed for the purpose of fabricating controlled microenvironments comprised of cells, nutrients, hydrogels, and soluble signaling molecules in a repeatable fashion. The first application explored was the development of a novel, bioprinted, cell-based biosensor as a nondestructive method for the monitoring of the cellular redox environment. Mammalian cells were engineered to express a redox sensitive protein and were patterned and immobilized within a photopolymerizing hydrogel matrix, resulting in biocompatible, three-dimensional microenvironments which supported cell growth and facilitated small molecule sensing. Exposure of the printed, redox sensitive cells to oxidative and reductive compounds and monitoring via confocal microscopy demonstrated proper and reversible functioning of the living biosensor. Bioprinting was also used to generate complex, micro-scale, multi-species populations of bacteria in order to evaluate the effects of distance and various forms of competition on syntrophic relationships. An artificial, syntrophic bacterial consortium was printed within controlled microenvironments confined by geometry and nutrient availability. The growth of the printed strains was monitored, analyzed, and compared to the predictions of an experimental, computational bacterial growth model known as COMETS. Results indicated that the general trends exhibited in vitro by most of the examined micro-scale interactions can be predicted in silico, and that the effects of microbial interactions on the micro-scale can differ considerably than those observed at the macro-scale.
    URI
    http://hdl.handle.net/1951/66926
    Collections
    • Colleges of Nanoscale Science and Engineering Doctoral Dissertations [5]

    SUNY Digital Repository Support
    DSpace software copyright © 2002-2022  DuraSpace
    Contact Us | Send Feedback
    DSpace Express is a service operated by 
    Atmire NV
     

     


    SUNY Digital Repository Support
    DSpace software copyright © 2002-2022  DuraSpace
    Contact Us | Send Feedback
    DSpace Express is a service operated by 
    Atmire NV