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dc.contributor.advisorMeng, Yizhien_US
dc.contributor.authorLiu, Gaojunen_US
dc.contributor.otherDepartment of Materials Science and Engineeringen_US
dc.date.accessioned2013-05-24T16:38:22Z
dc.date.available2013-05-24T16:38:22Z
dc.date.issued1-May-12en_US
dc.date.submitted12-Mayen_US
dc.identifierStonyBrookUniversityETDPageEmbargo_20130517082608_116839en_US
dc.identifier.urihttp://hdl.handle.net/1951/60289
dc.description51 pg.en_US
dc.description.abstractIn the last decade, non-viral polymeric vectors have become more attractive than their viral counterparts due to their nontoxicity and good biocompatibility. However, one of the major drawbacks is the low transfection efficiency when compared to viruses. In this work, a naturally cationic polysaccharide, chitosan, was cross-linked with negatively charged tripolyphosphate (TPP) to synthesize chitosan/TPP nanoparticles (CNPs) for delivery of plasmid DNA (pDNA). Particle size and zeta potential were characterized for CNPs with chitosan-TPP mass ratios of 4:1 and 6:1 (w/w) using benchtop dynamic light scattering. And both potentiometric titration method and FTIR spectrometer were applied to measure the degree of deacetylation of chitosan. Release kinetics of a model protein (bovine serum albumin, BSA) showed a steady release that reached 7% after 6 days. Besides that, we also assessed the in vitro transfection efficiency of the CNP- pDNA system using fluorescence microscopy, as well as the effect of tumor suppressant p53. Later the release kinetics and encapsulation efficiency of plasmid DNA bound to the CNPs will be investigated. Additionally, we will try to improve the gene transfection efficiency in both MC3T3-E1 and osteosarcoma cells by applying Sonicator 740 therapeutic ultrasound.en_US
dc.description.sponsorshipStony Brook University Libraries. SBU Graduate School in Department of Materials Science and Engineering. Charles Taber (Dean of Graduate School).en_US
dc.formatElectronic Resourceen_US
dc.language.isoen_USen_US
dc.publisherThe Graduate School, Stony Brook University: Stony Brook, NY.en_US
dc.subject.lcshMaterials Science--Biomedical engineeringen_US
dc.subject.otherchitosan/TPP nanoparticles, gene therapy, ionic gelation, non-viral gene vector, p53en_US
dc.titleChitosan/TPP Nanoparticles as a Gene Delivery Agent For Tumor Suppressant P53en_US
dc.typeThesisen_US
dc.description.advisorAdvisor(s): Meng, Yizhi . Committee Member(s): Meng, Yizhi ; Gersappe, Dilip ; Hadjiargyrou, Michael.en_US
dc.mimetypeApplication/PDFen_US


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