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dc.contributor.advisorKrug, Laurie Ten_US
dc.contributor.authorReddy, Sandeep Steven-Andrewen_US
dc.contributor.otherDepartment of Biochemistry and Cell Biologyen_US
dc.date.accessioned2013-05-22T17:35:26Z
dc.date.available2013-05-22T17:35:26Z
dc.date.issued1-May-12en_US
dc.date.submitted12-Mayen_US
dc.identifierReddy_grad.sunysb_0771M_10962en_US
dc.identifier.urihttp://hdl.handle.net/1951/59835
dc.description57 pg.en_US
dc.description.abstractThe human gammaherpesviruses (gammaHV), Kaposi's sarcoma-associated virus (KSHV) and Epstein-Barr virus (EBV), are the etiologic agents of many lymphoproliferative and neoplastic disorders. Murine gammaherpesvirus 68 (MHV68) has a high biological and genetic correlation to EBV and KSHV and represents an amenable, tractable model system to investigate host-virus interactions during chronic gammaHV infections. Modulating host cellular gene expression is one common strategy utilized during infection by all three viruses. Here, we have shown that four previously reported housekeeping genes (HKGs), ActB, beta2M, GAPDH and HPRT, evade host shutoff and exhibit stable transcript expression. The validation of these genes in the context of MHV68 infection allowed us to normalize the transcriptional expression of viral ORF50, an immediate early gene, by RT-qPCR. The validation of these four genes will allow us to identify additional HKGs with similar attributes that escape virally induced host shutoff. The compilation of these validated and novel HKGs can then be applied as reference genes to define the transcriptome of gammaherpesvirus infections in multiple cell lines and phases of the viral lifecycle.en_US
dc.description.sponsorshipStony Brook University Libraries. SBU Graduate School in Department of Biochemistry and Cell Biology. Charles Taber (Dean of Graduate School).en_US
dc.formatElectronic Resourceen_US
dc.language.isoen_USen_US
dc.publisherThe Graduate School, Stony Brook University: Stony Brook, NY.en_US
dc.subject.lcshBiochemistry--Molecular biology--Geneticsen_US
dc.subject.othergammaherpesvirus, HKG, housekeeping genes, MHV68, murine gammaherpesvirus 68en_US
dc.titleIdentification and Validation of References Genes for Transcriptome Analysis of Murine Gammaherpesvirus 68 Infectionen_US
dc.typeThesisen_US
dc.description.advisorAdvisor(s): Krug, Laurie T. Committee Member(s): Carpino, Nicholas.en_US
dc.mimetypeApplication/PDFen_US


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