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dc.contributor.advisorGorfinkel, Veraen_US
dc.contributor.authorGudkov, Dmytroen_US
dc.contributor.otherDepartment of Electrical Engineeringen_US
dc.date.accessioned2013-05-22T17:34:40Z
dc.date.available2013-05-22T17:34:40Z
dc.date.issued1-Dec-11en_US
dc.date.submitted11-Decen_US
dc.identifierGudkov_grad.sunysb_0771M_10786en_US
dc.identifier.urihttp://hdl.handle.net/1951/59675
dc.description66 pg.en_US
dc.description.abstractI present a novel single photon spectrometer and discuss its use for ultra-fast and highly accurate detection of quantum dots and micro-beads encoded with compositions of various types of quantum dots. The spectrometer receives a polychromatic light through a fiber input. The received light undergoes color decomposition by a diffraction grating and is further detected by a 32-channel PMT (Hamamatsu, Japan) operating in single photon counting mode. Due to a novel, highly advanced analog/digital circuitry operating in GHz range, the 32-channel single photon detector has a unique performance: it supports detection of up to 108 photon/s/channel, data acquisition rate at up to 106 frames/s, data transfer and via Ethernet, and data recording speed up to 32MB/s using a standard PC. Due to an extremely broad linearity range and high data acquisition speed, the developed spectrometer has been successfully used for the detection of fluorescent radiation from both single quantum dots and micro-beads with embedded quantum dots of different colors. Our experiments demonstrated the ability of the spectrometer to detect up to 10,000 micro-particles per second. For the beads with embedded quantum dots of 6 different colors we were able to distinguish up to 105 different color combinations (QDs fluoresce in spectral range between 490nm and 700nm). Currently, in the field of molecular biology there is a wide variety of techniques and assays based on color labeled micro- and nano-particles. The unique detection performance of our single photon spectrometer suggests its successful use in multiple applications such as life sciences, molecular diagnostics, personalized medicine and others.en_US
dc.description.sponsorshipStony Brook University Libraries. SBU Graduate School in Department of Electrical Engineering. Charles Taber (Dean of Graduate School).en_US
dc.formatElectronic Resourceen_US
dc.language.isoen_USen_US
dc.publisherThe Graduate School, Stony Brook University: Stony Brook, NY.en_US
dc.subject.lcshElectrical engineeringen_US
dc.subject.otherfluorescence detection, quantum dots, single photon counting, Spectrometeren_US
dc.titleDevelopment of a single photon detector for fluorescent spectrometryen_US
dc.typeThesisen_US
dc.description.advisorAdvisor(s): Gorfinkel, Vera . Committee Member(s): Donetski, Dmitri.en_US
dc.mimetypeApplication/PDFen_US


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