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dc.contributor.advisorReich Marshall, Nancy C, Krug, Laurieen_US
dc.contributor.authorEspaillat, Mel Pilaren_US
dc.contributor.otherDepartment of Biochemistry and Cell Biologyen_US
dc.date.accessioned2013-05-22T17:34:30Z
dc.date.available2013-05-22T17:34:30Z
dc.date.issued1-Aug-12en_US
dc.date.submitted12-Augen_US
dc.identifierEspaillat_grad.sunysb_0771M_11069en_US
dc.identifier.urihttp://hdl.handle.net/1951/59643
dc.description49 pg.en_US
dc.description.abstractInterferons (IFNs) are the most potent mediators of antiviral activities in the immune system. Viral molecular patterns are detected by receptors of the innate immune system, resulting in IFN production and subsequent IFN-stimulated gene (ISG) expression. The production of IFN serves as a danger signal that triggers a cascade of molecular events in the cell for the induction of antiviral mechanisms. The ISG products protect cells against viral replication, induce anti-proliferative mechanisms, and modulate the development of innate and adaptive immunity. The expression of ISG54 is mediated by type I IFN signaling and results in inducement of apoptosis via a mitochondrial pathway. To understand the molecular mechanism underlying this activity, we aimed to identify molecular partners of the gene product, p54. Two antioxidant enzymes were identified from a yeast two-hybrid assay, peroxiredoxin 5 (PRDX5) and superoxide dismutase 2 (SOD2). To confirm the interaction between p54 and both proteins, we cloned the cDNA of both genes and confirmed their interaction with p54 by co-immunoprecipitation and Western blot. In addition, we tested if these antioxidants could regulate ISG54-mediated apoptosis. Apoptosis was assessed using flow cytometric analysis of cells co-transfected with ISG54 with or without PRDX5 and SOD2 and co-stained with Annexin-V and propodium iodide (PI). In this report we show that both enzymes can transiently inhibit ISG54-mediated apoptosis. The results describe novel findings in the molecular mechanism of ISG54 cellular activity.en_US
dc.description.sponsorshipStony Brook University Libraries. SBU Graduate School in Department of Biochemistry and Cell Biology. Charles Taber (Dean of Graduate School).en_US
dc.formatElectronic Resourceen_US
dc.language.isoen_USen_US
dc.publisherThe Graduate School, Stony Brook University: Stony Brook, NY.en_US
dc.subject.lcshBiochemistry--Cellular biologyen_US
dc.titleRegulation of ISG54-mediated Apoptosis by Antioxidants PRDX5 and SOD2en_US
dc.typeThesisen_US
dc.description.advisorAdvisor(s): Reich Marshall, Nancy C; Krug, Laurie. Committee Member(s): Dean, Neta.en_US
dc.mimetypeApplication/PDFen_US


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