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dc.contributor.authorApostol, Monicaen_US
dc.contributor.otherDepartment of Materials Science and Engineeringen_US
dc.date.accessioned2012-05-17T12:19:46Z
dc.date.available2012-05-17T12:19:46Z
dc.date.issued1-May-11en_US
dc.date.submittedMay-11en_US
dc.identifierApostol_grad.sunysb_0771E_10421.pdfen_US
dc.identifier.urihttp://hdl.handle.net/1951/55947
dc.description.abstractWe have found that P4VP and PMMA thin films can be etched with UVA radiation. Furthermore, we also found that dermal fibroblasts could be cultured successfully on the P4VP polymer, with a doubling time comparable to tissue culture Petri dish standards. Consequently we were able to grow tissue on P4VP substrates which could easily be lifted using UVA radiation. The cells that were removed were then re-plated at a lower density and a series of assays was performed at 3 and 6 days. While only a small amount of damage was discernable at day 3 nearly complete recovery was observed at day 6. The technique was also used to pattern areas within the tissue, where other types of cells could be inserted. In order to demonstrate the technique, a hybrid tissue layer was produced, where the dermal fibroblasts in a circular area at the center of the sample were removed via exposure through a mask. A keratinocyte layer was inserted which adhere to the fibroblast layer forming a tissue with integrated layers of two distinct cell types. We also investigated the effects of coated TiO2 particles on cells exposed to UVC. We found that as expected, cells were adversely affected by exposure to UVC and died even after exposure to as little as 3.5 J/cm2. Addition of 0.4mg/ml TiO2 particles that were uncoated did not provide protection, and the cells died at the same rate. Addition of 4mg/ml of coated TiO2 on the other hand, did not affect cell viability in the absence of UV light and increased the viability after exposure to UVC radiation. In fact the cells containing the iv coated particles were indistinguishable for the unexposed control samples even after exposure to as much as 7.1J/cm2 of UVC.en_US
dc.description.sponsorshipStony Brook University Libraries. SBU Graduate School in Department of Materials Science and Engineering. Lawrence Martin (Dean of Graduate School).en_US
dc.formatElectronic Resourceen_US
dc.language.isoen_USen_US
dc.publisherThe Graduate School, Stony Brook University: Stony Brook, NY.en_US
dc.subject.lcshMaterials Science -- Biologyen_US
dc.subject.otherbiomaterials, cells, photo-sensitive polymersen_US
dc.titlePhoto Cleavable Polymers for Tissue Engineeringen_US
dc.typeDissertationen_US
dc.description.advisorAdvisor(s): Miriam Rafailovich. Vladimir Jurukov. Committee Member(s): Miriam Rafailovich; Vladimir Jurukov; Nadine Pernodet; Liliana George.en_US
dc.mimetypeApplication/PDFen_US


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