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dc.contributor.advisorStillman, Bruceen_US
dc.contributor.authorRossmann, Marlies Petraen_US
dc.contributor.otherDepartment of Geneticsen_US
dc.date.accessioned2012-05-15T18:06:36Z
dc.date.available2012-05-15T18:06:36Z
dc.date.issued1-Aug-10en_US
dc.date.submittedAug-10en_US
dc.identifierRossmann_grad.sunysb_0771E_10275.pdfen_US
dc.identifier.urihttp://hdl.handle.net/1951/55606
dc.description.abstractIn budding yeast, telomere position effect variegation (TPEV) was discovered when prototrophic markers were placed near chromosome ends and was interpreted to reflect a reversible form of heterochromatin. Selection for or against these markers demonstrated roles for several proteins in telomeric heterochromatin formation; these include the SIR protein complex, chromatin assembly factors (CAF-1, Asf1), PCNA as a DNA replication factor as well as DNA damage checkpoint proteins. PCNA (POL30) in particular as a component of the core DNA replication machinery has been shown to link DNA replication to the inheritance of nucleosomes and was, by extension, proposed to help maintain silenced chromatin. In analyzing the phenotype of the silencing defective pol30-8 mutant using various TPEV reporter strains I found that this mutant exhibits only a very subtle telomeric silencing defect in comparison to a sir3&Delta mutant. Furthermore, employing the common URA3 reporter at the telomere of chromosome VIIL that can be counter-selected with 5-FOA in a genetic screen, I identified high-copy suppressors of the pol30-8-dependent silencing defect. Interestingly, one of the suppressors, CDC21, the thymidylate synthase gene, also counteracted the telomeric silencing defect of a strain deleted for DOT1, encoding the only histone H3K79 methyltransferase in S. cerevisiae. Gene expression analysis of pol30-8 mutant and dot1 mutant strains surprisingly revealed that dot1&Delta deletion results in repression of telomeric gene expression without an effect on Sir2/4 occupancy. On the other hand, the pol30-8 mutation was linked to a general up-regulation of normally poorly expressed genes. Notably, the effect of pol30-8 correlated with decreased histone levels. Among the affected genes were the ribonucleotide reductase (RNR) genes whose expression could be further induced by treatment with 5-FOA. Importantly, inhibition of RNR activity as well as mutations in the RAD53 DNA damage response pathway rescued the sensitivity of pol30-8 URA3-VIIL cells to 5-FOA. I speculate that in the context of low URA3 expression such as in a URA3-VIIL strain a misbalance between Ura3, RNR and Cdc21 activity is responsible for a higher conversion rate of 5-FOA into its toxic metabolites which accounts for the 5-FOA sensitivity seen in pol30-8 and dot1&Delta URA3-VIIL mutants. In conclusion, while I found that Pol30 facilitates normal histone distribution with consequences for global gene expression, neither pol30-8 nor dot1&Delta mutants are defective in telomeric heterochromatin formation.en_US
dc.description.sponsorshipStony Brook University Libraries. SBU Graduate School in Department of Genetics. Lawrence Martin (Dean of Graduate School).en_US
dc.formatElectronic Resourceen_US
dc.language.isoen_USen_US
dc.publisherThe Graduate School, Stony Brook University: Stony Brook, NY.en_US
dc.subject.lcshBiology, Genetics -- Biology, Molecular -- Biology, Microbiologyen_US
dc.subject.otherbudding yeast, DNA damage response, DNA replication, heterochromatin, nucleotide metabolism, telomeric silencingen_US
dc.titleA common telomeric gene silencing assay is affected by nucleotide metabolism and the DNA damage response: implications for the role of PCNA in heterochromatin formation.en_US
dc.typeDissertationen_US
dc.description.advisorAdvisor(s): Bruce Stillman. Committee Member(s): Nancy Hollingsworth; Bruce Futcher; Rolf Sternglanz; Rodney Rothstein.en_US
dc.mimetypeApplication/PDFen_US


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