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dc.contributor.advisorSmolka, Scott A.; Grosu, Raduen_US
dc.contributor.authorHuang, Wei Chiehen_US
dc.contributor.otherDepartment of Physiology and Biophysicsen_US
dc.date.accessioned2012-05-15T18:04:09Z
dc.date.available2012-05-15T18:04:09Z
dc.date.issued1-Aug-10en_US
dc.date.submittedAug-10en_US
dc.identifierHuang_grad.sunysb_0771E_10263.pdfen_US
dc.identifier.urihttp://hdl.handle.net/1951/55474
dc.description.abstractTear secretion is a complicated process that involves two different cell types. The lacrimal acinar cell is thought to secrete a hypertonic primary fluid with a high K<super>+</super> concentration. This fluid is then modified by the ductal epithelia and transported to the avascular ocular surface. It is known that both sympathetic and parasympathetic input trigger protein release, but the role of adrenergic stimulation on water secretion in lacrimal gland remains unclear. Therefore, the first goal of this dissertation is to examine the influence of&#913;- and&#914;-adrenergic activation on tear fluid production.Tear secretion requires coordination of different membrane channels and transporters. Walcott et al. showed the importance of basolateral Na<super>+</super>-K<super>+</super>-2Cl<super>-</super>-cotransporter (NKCC1) on secretion rate. The second goal is to develop an acinar cell mathematical model with cell volume regulation. The model is used to examine whether NKCC1 alone is sufficient to generate hypertonic tear fluid with high KCl content when the cell is stimulated. Subsequently the model was expanded with acid-base regulatory transporters to investigate the interaction between volume- and pH-regulation systems. This effort led to the discovery that the sodium-bicarbonate transporter is expressed in lacrimal gland and participates in tear production.Lastly, since acinar and duct cells express same membrane channels and transporters, it is likely that duct cells secrete a hypertonic fluid like acinar cell. The third goal of this project is to develop a duct cell model and combine it with the acinar cell model. The combined model was used to examine the flow dependence of tear composition. The predictions agree with measurements made in anesthetized rats.en_US
dc.description.sponsorshipStony Brook University Libraries. SBU Graduate School in Department of Physiology and Biophysics. Lawrence Martin (Dean of Graduate School).en_US
dc.formatElectronic Resourceen_US
dc.language.isoen_USen_US
dc.publisherThe Graduate School, Stony Brook University: Stony Brook, NY.en_US
dc.subject.lcshBiology, Physiologyen_US
dc.subject.otherLacrimal Gland, Mathematical Model, Water and Electrolyte Secretionen_US
dc.titleWater and Ion Transport in Lacrimal Gland: Models and Experimenten_US
dc.typeDissertationen_US
dc.description.advisorAdvisor(s): Leon C. Moore. Committee Member(s): Peter R. Brink; Chris Clausen; Peter Reinach.en_US
dc.mimetypeApplication/PDFen_US


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